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Digital and Video Image Examination Approaches:
Since microscopes are visual tools, there has long
been a desire to get the image out of the microscope and into a form that
can be easily shared. Traditionally this has taken the form of 35mm or
Polaroid film micrography. In the last few years, however, there has been
a profound shift from film-based cameras to video and, more recently,
digital micrography.
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Which is better: Film, Video, or Digital cameras?
The answer depends on what your goals are. Generally, for the highest
resolution for the least cost, film cameras are still the solution. We
can mount most 35mm SLR type cameras to most trinocular microscopes very
easily. Or, dedicated photomicrography systems are readily available, but
can be very expensive. It has been estimated that 35mm film is the
equivalent of a 15 megapixel (+/-) digital image. Digital cameras have
progressed considerably in the last couple of years to the point that 5- 6
megapixel cameras are no longer uncommon, but these still fall short.
However, there are other considerations besides image
resolution. The ability to transmit images to a group on a television
monitor or over the internet in many cases makes up for a lack of
resolution. For instance, a 3 megapixel digital camera will produce up to
a 5"x7" print at 300dpi, while a 35mm negative can produce up to, say, a
16"x20" print. So, the question becomes, how often do you need to enlarge
a print beyond 5"x7"? If the answer is never, a digital camera is a good
choice! Here's a chart of common digital camera resolutions, the
corresponding print sizes at 300dpi, and an example camera:
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| 0.3 Megapixel |
640 x 480 pixels |
1.6" x 2.13" print size at 300dpi |
Hitachi VKC370W Video Camera |
| 1.3 Megapixel |
1280 x 960 pixels |
3.2" x 4.3" print size at 300dpi |
Sony Mavica FD92 |
| 2.1 Megapixel |
1600 x 1200 pixels |
4" x 5.3" print size at 300dpi |
Polaroid DMC2 |
| 3.3 Megapixel |
2048 x 1536 pixels |
5.1" x 6.8" print size at 300dpi |
Nikon Coolpix 995, Sony F505 |
| 5.2 Megapixel |
2560 x 1920 pixels |
6.4" x 8.5" print size at 300dpi |
Sony Cybershot F707 |
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Digital vs. Video: Video cameras are the older
of these technologies, but they still have the advantage of outputting
"live" (30 frames / second) images, which digital still cameras, due to
their higher resolutions, can't do - at least not at full resolution
(but see the new PixeLINK camera below as an exception). Both use
electronic sensors (CCD's or CMOS chips) to convert light into analog
electrical signals. A digital camera is one which converts the analog
signal to digital within the camera and outputs a digital signal.
Confusion occurs because many analog video cameras use "digital signal
processing" (DSP). These cameras process the signal digitally, but still
output an analog signal. To add to the confusion, analog video signals
can be converted to digital inside a computer using a frame grabber. This
is a good solution for those who want to view live video, for a classroom
demonstration or training seminar, but who also want to be able to capture
digital still images for presentations, inclusion in report documents,
e-mailing, etc. When converted to digital, video camera images are
typically 640x480 pixels (0.3 megapixels). While this is no longer
state-of-the- art, it is good enough for lots of applications - especially
if the image can stay in an electronic format. Below is a full-size
640x480 image taken using a video camera / frame grabber package:
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In summary, digital image capture is the most rapidly improving
technology, while video and film are more slowly evolving. Digital image
capture has the promise of greater speed, greater economy, greater
flexibility and ease of use.
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